Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDRIREKLQILADAAKYDVSCSSSGSDRKNKNKGLGDASHSGICHSYTEDGRCVSLLKILFSNVCIFDCAYCVSRRSNDVQRAAFTVQEVVDLTINFYRRNYIEGLFLS-SGIF-KSAD-HTMERMLQVVKKLRL-EENFN---GYIHLKTIPGASPELIHEAGL-Y--ADRMSINLEMPTEIGLKTFAPEKSHQEVQKDLGLVRDRLIQLKDERQIIKHVPKYVPAGQTTQMVVGAHQESDQDVLFMADKHYKEFKLKRVYFSGYIPINNENNYLPAVGSAPPLLRENRLYQSDWLMRFYGFEVNEIVNEKHPNLDLDVDPKLSWALRHPEQFPVDLNRADYQMILRVPGIGVKSAKKIVQARRFGKIHIDLLKKLGVAYQRAKFFIRCEDSPKFQKELSSSFIRQQILTQGSSKYVQQLSPQLSLGF 4JC0 Chain:A ((138-337)) -------------------------------------------------------PYAYVKISDGCDRGCTFCSIPSFKGSLR-SRSIEDITREVEDLLKE-GKKEIILVAQDTTSYGIDLYRKQALPDLLRRLNSLNGEFWIRVMYLHPD---HLTEEIISAMLELDKVVKYFDVPVQHGSDKILKLMGRTKSSEELKKMLSSIRER----------------FPDAVLRTSIIVGFPGETEEDFEELKQFVEE-IQFDKLGAFVYSDEEG-TVAFN--------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 902 5398 5.98 28.41 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 5.98 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_4JC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JC0-query.scw PDB file : Tito_Scwrl_4JC0.pdb: