Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYQFEAELLVGDIKQFADYKGKVLLIVNTASKCGFTP-QFAGLEKLYEKYKDLGLEVLGFPCNQFGGQDPGSNKEIGTFCQRN-YGVKFPMFAKVDVKGPEAHVIFRYLTREAKGILGSSTIKWNFTKFLVGKDGSVLNRYAPTTKPEALEADIEKALAS 2P5Q Chain:A ((10-168)) -ESVHDFTVKDAKENDVDLSIFKGKVLLIVNVASKCGMTNSNYAEMNQLYEKYKDQGLEILAFPCNQFGEEEPGTNDQITDF-VCTRFKSEFPIFDKIDVNGENASPLYRFLKLGKWGIFGD-DIQWNFAKFLVNKDGQVVDRYYPTTSPLSLERDIKQLLE-

General information: TITO was launched using: RESULT: Template: 2P5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 791 -12962 -16.39 -82.56 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -16.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2P5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P5Q-query.scw PDB file : Tito_Scwrl_2P5Q.pdb: