Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHFQFQTVPNIISGLGSIQELQNVLASKTYRKLLLVTDAGMI--KNQLHLPILQILDTLKIEHVIYADVQADPPEQVVLDAANYAKQQNVDVIVGFGGGSSLDVAKVIALLAHPNQTQNLQEMYGVNQVKTARLPLILIPTTAGTGSEVTPISIVTTGETT-KMGIVSPVLYADTAILDATFTQNLPAHITAATGIDAMVHAIEAYTSKHKKNIYTDILAKHALKLLNHNLPKVLKNGNDLEARQNMLFGSMLAGQAFANSPVAAVHALAYPLGGHFHLSHGHTNALVLVEVLKFNAPYAKQYYAELMQWLDPYSNGST-DGLCDLFIDHMQNHLDRSGLTLKLSDLGIEESSLPRLAQDAMLQTRLLQNNPRDMTETAALAIYQAIY 4FR2 Chain:A ((16-398)) --AYDFLMPSVNFFGPGVISKIGERAKMLGMKKPVIVTDKFLENLKNGAVAQTLASLKKSGVDYVVYNGVEPNPKIHNIKEVKTLYEKEDADSIITVGGGSAHDTGKGAGIIMTN--GDDITKLAGIETLKNPLPPLIAVNTTAGTGSELTRHAVITNEETHLKFVVVSWRNIPLVSFNDPTLMLDIPKGLTAATGMDAFVQAVEPYVSVD-HNPITDSQCIQAIKLIESSLREAVANGHNLQARTKMVEAEMLAGMAFNNANLGYVHAMAHQLGGQYDAPHGVCCALLLPYAEEYNLIADPERFAELARIMGENTDGLSTRDAAELSIKAMKQLSEDVGIPHSIKDIGAKPEDFDLMAENALKDG-NAFSNPRKGTKEDIVKIFQEAY

General information: TITO was launched using: RESULT: Template: 4FR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2323 -60205 -25.92 -158.85 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -25.92 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4FR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FR2-query.scw PDB file : Tito_Scwrl_4FR2.pdb: