Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKATE--YIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAG----ASER-V----------DVE--------VEKKHPFDFVLWKH--------AKEN----EPSW-ASPWGNGRPGWHIECSAMS------TCC---LG-----------NHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWIHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSS-HYRSPVNFSDVALKEAK--TSLTRFYHSFKAYQQVYGQ----------------TTTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQAD--------QATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 5K0S Chain:C ((25-514)) ----------------------EKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQSARKEGITPRDLADRNTSAFRRMAEVLNSS-NDDYIRTSEERHYKASQAIWQAMVANGDIYKGG-YAGWYSVRDE-AY--YGEEETEVRADGVRYGPQGTPVEWVEEESYFFRLSAYQDKLLDLYENNP-GFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGD--EKHVMYVWVDALTNYITALGYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSA-QLPLPKRVFAHGFLFNR-----------IDPFELVERYGLDQLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETARKAMD-DQALHLALGAIFAVVAEANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPF---------------IPQSAEKLLDILAVPADKRQF--ADVLASPLA-------------------

General information: TITO was launched using: RESULT: Template: 5K0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1836 -39028 -21.26 -99.06 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -21.26 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_5K0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K0S-query.scw PDB file : Tito_Scwrl_5K0S.pdb: