Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLIRKAKKDDTDTIVAIIAPYVDQVIANDEGRSRFKSEAIQTIFD--REDIHYFVGEIDQQIVGIVAYME----------PSHLMHFFIKKTHLKLGLGRQLWDFIEEKIKNENIDIEKITVNS-SFYAQDIYEKFGFIVSGDAAEK-WGIRFIPMTKYYALASEK 3F5B Chain:A ((14-176)) -RFCFKQMNKSQHELVLGWIHQPHINEWLHGDGL-SNTIKDLHEFLNDGKPWATHWIAYDNEIPFAYLITSEIEKSEEYPDGAVTLDLFICRLDYIGKGLSVQMIHEFILSQF-SDTKIVLINPEISNERAVHVYKKAGFEIIGEFIASWHPVPHYKMKLCIEDLK--

General information: TITO was launched using: RESULT: Template: 3F5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 579 -36214 -62.55 -243.05 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -62.55 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_3F5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F5B-query.scw PDB file : Tito_Scwrl_3F5B.pdb: