Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWNPNSIIFCFYFIFSILALWGIIEAWVHQSRTETIHPFKAFVHLLAFYLSYLLIPLWFFNLYAGWIGYYSIHETIFIFLLSGILIYARFIEPHMVRVDVHQYRLNPDRHFVKPVKVALIADLHIGLFSGHERQLKIIVKKLNEQQPDLVVVAGDWTYEPED--KLVQELSVLKDIKAPVYSVPGNHDEQYPGPPIQQLLKDALYY----NDVMDIEGKIVEFD-EFRLIGIGDLWAG-----KT----DMRSMPD-----L--PQDKPWLILSHNPDTVDMV-----------PKLPNRPLMLS---GHTHGGQVELPLVTNYIMKKVSILGHKRGFYSHEHADVFVTVGTGMVGIPLRFRVPPTIDIIELA 1UF3 Chain:G ((3-195)) ---------------------------------------------------------------------------------------------------------------RTVRYILATSNPMGDL-----EALEKFVKLAPDTGADAIALIGNLMPKAAKSRDYAAFFRILSEAHLPTAYVPGPQDAPIWEY-----LREAANVELVHPEMRNVHETFTFWRGPYLVAGVGGEIADEGEPEEHEALRYPAWVAEYRLKALWELKDYPKIFLFHTMPYHKGLNEQGSHEVAHLIKTHNPLLVLVAGKGQKHEM-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1UF3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 775 -16936 -21.85 -108.56 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain G : 0.53 3D Compatibility (PKB) : -21.85 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1UF3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UF3-query.scw PDB file : Tito_Scwrl_1UF3.pdb: