Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEELLNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILKSNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDIQYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKKTREVTLANGETIPASIRGLKKLIS 1L5Y Chain:B ((3-110)) APSVFLIDDDRDLRKAMQQTLELAG------FTVS-SFASATEALAGLSAD--FAGIVISDIRMPG--MDGLALFRKILALDPDLPMILVTGHGDIPMAVQAIQDGAYDFIAKPFAADR---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1L5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 500 -68451 -136.90 -633.81 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -136.90 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_1L5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L5Y-query.scw PDB file : Tito_Scwrl_1L5Y.pdb: