Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----GGECIVKLHDPN--GKVVSWFIALAGKREVKKEAAFSWSCSTNSET------CEAEIPLCIVPKNFKPV-GEHYKGETPKVAT 1FNM Chain:A ((404-482)) VPEPVIDVAIEPKTKADQEKLS---QAL---ARLAEEDP-TFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDANVGKPQVA-

General information: TITO was launched using: RESULT: Template: 1FNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 18774 94.34 284.45 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 94.34 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_1FNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNM-query.scw PDB file : Tito_Scwrl_1FNM.pdb: