Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLSLVLAAFCLGIASAVPKFDQNLDTKWYQWKATHRRLY-GANEEGWRRAVWEKNMKMIELHNGEYSQGKHGFTMAMNAFGDMTNEEFRQMMGCFRNQKFRKGKV-FREPLFLDLPKSVDWRKKGYVTPVKNQKQCGSCWAFSATGALEGQMFRKTGKLVSLSEQNLVDCSRPQGNQGCNGGFMARAFQYVKENGGLDSEESYPYVAVDEICKYRPENSVANDTGFTVVAPGKEKALMKAVATVGPISVAMDAGHSSFQFYKSGIYFEPDCSSKNLDHGVLVVGYGFEGANSNNSKYWLVKNSWGPEWGSNGYVKIAKDKNNHCGIATAASYPNV 3QT4 Chain:A ((20-329)) ---------------------KSLFQEQWSQFKLTHKKSYSSPIEEIRRQLIFKDNVAKIAEHNAKFEKGEVTYSKAMNQFGDMSKEEFLAYVNRGKAQ----NLRMPYVSSKKPLAASVDWRS-NAVSEVKDQGQCGSSWSFSTTGAVEGQLALQRGRLTSLSEQNLIDCSSSYGNAGCDGGWMDSAFSYIHDY-GIMSESAYPYEAQGDYCRFDSSQSVTTLSGYYDLPSGDENSLADAVGQAGPVAVAIDAT-DELQFYSGGLFYDQTCNQSDLNHGVLVVGYGSD----NGQDYWILKNSWGSGWGESGYWRQVRNYGNNCGIATAASYPAL

General information: TITO was launched using: RESULT: Template: 3QT4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1857 64695 34.84 214.22 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 34.84 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3QT4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QT4-query.scw PDB file : Tito_Scwrl_3QT4.pdb: