Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRWILFIGALIGSSICGQEKFFGDQVLRINVRNGDEISKLSQLVNSNNLKLNFWKSPSSFNRPVDVLVPSVSLQAFKSFLRSQGLEYAVTIEDLQALLDNEDDEMQHNEGQERSSNNFNYGAYHSLEAIYHEMDNIAADFPDLARRVKIGHSFENRPMYVLKFSTGK--GVRRPAVWLNAGIHSREWISQATAIWTARKIVSDYQRDPAITSILEKMDIFLLPVANPDGYVYTQTQ--NRLWRKTRSRNPGSSCIGADPNRNWNASFAGKG-ASDNPCSEVYHGPHANSEVEVKSVVDFIQKH-----GNFKGFIDLHSYSQLLMYPYGYSVK-KAPDA-EELDKVARLAAKALASVSGTEYQVGPTCTTVYPASGSSIDWAYD-NGIKFAFTFELRDTG-TYGFLLPANQIIPTAEETWLGLKTIMEHVRDNLY 4IHM Chain:A ((5-315)) ----------------------------------------------------------------------------------------------------------------------YDSGYHNYNEMVNKINTVASNYPNIVKKFSIGKSYEGRELWAVKISDNVGTDENEPEVLYTALHHAREHLTVEMALYTLDLFTQNYNLDSRITNLVNNREIYIVFNINPDGGEYDISSGSYKSWRKNRQPNSGSSYVGTDLNRNYGYKWGCCGGSSGSPSSETYRGRSAFSAPETAAMRDFINSRVVGGKQQIKTLITFHTYSELILYPYSYTYTDVPSDMTQDDFNVFKTMANTMAQTNG--YTPQQGS-DLYIADGGMD-WAYGQHKI-FAFTFEMYPTSYNPGFYPPDEVIGRETSRNKEAVLYVAEKADCP--

General information: TITO was launched using: RESULT: Template: 4IHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 14893 8.78 50.31 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 8.78 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_4IHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IHM-query.scw PDB file : Tito_Scwrl_4IHM.pdb: