Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 2RVQ Chain:D ((4-129)) MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

General information: TITO was launched using: RESULT: Template: 2RVQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 195 -30802 -157.96 -244.46 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 1.00 3D Compatibility (PKB) : -157.96 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_2RVQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RVQ-query.scw PDB file : Tito_Scwrl_2RVQ.pdb: