Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALSLEEFVYSL--------DLRTLPRVLEIQSGIYFEGS------VYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFY---HLKDIKLENLIIKQGEPIRFNSVEEINGET--LVNCGV---VRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLK--------FQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKA-FHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGG-----FVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSI----KDDILAATPGLQLEEDITDSYLLI----SDFANPEECWE--IPMSRLNMTVRLVNGSSLPA---DAGLLQVRSFVEE-------ITEEQYYMMRRYES------SLSH-------PPPRPPKHPSAEEMK--LTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQ-----PQAPL------- 5L9W Chain:A ((2-684)) AIPTLEQKLTWLKPAPASSRELDLAAQIDPAQFEIGFQRTNDILDEGMDVFVRSCRCAMG-VAGDSLVAIMTADGDIVNGSCGT------YLHAVIPPLIIKYILETYGDE--------IRDGDLWFANDAVYGGVHNPDQMVCMPVYYEGKLVAWTAALVHTTETGAIEPGGMPVSATTRFEEGMNLPPMRIGENFKLREDVVSMFVAFG------LRAPSMIAVDLKARCTTADRVRTRIIELCEREGADYVTGLFRKMLQVAEAGARELIEQWPDGKYRCVTFSDAVG---LKQGLVRSCYMTLEKKGDRMLVDLSETGPETPSPYNA-HPQAAIAHFSNYIYEYLFHSLPISNGTFANIDFKFGKNTCLSPDPR-AATSCSVMISTGVMSAVHNACAKAMFSTSLWKQSGASMGNGGNA-------LVLAGQNQWGSSFADMLAYSINTEGQGARPTEDGMDAFGFPWCVFGRAP---NTESVENEFPLLVPLSNHWKDSCGHGKYRGGVGTAQVWVAHHVPELYMMAIADNTKLQTPQPLFGGYAPCTVPGIGIRNANIKELMAEGSDKIKLDVETLLAERTIDGKYEIEFQGRSVRPYSNGEVVTFAFSCGGTGYGDPLDRDPKSVEVDLLKGVLTEQTAQNIYKVKWDANLRRVDLDETSRLRAAEHDARRKRGVPYEQFEREWLKQRPDDEILKYYGTWPDAKVAQPLLRA

General information: TITO was launched using: RESULT: Template: 5L9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4130 90493 21.91 150.82 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 21.91 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.088

(partial model without unconserved sides chains): PDB file : Tito_5L9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L9W-query.scw PDB file : Tito_Scwrl_5L9W.pdb: