Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFRN-RILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH 4GKW Chain:A ((250-407)) EDKEDEVADLKQDTE-SLQKQLEENQEELEIVGNMLREEQGKVDQLQKRNVAHQKEIGKLRAELGTAQRNLEKADQLLKRNSQQQNQQSLDMRKLGELEADLKEKDSMVES---LTETIGILRKELENEKLKAAENMDSFEKLSMENENLKEKIAHYRAQRF---

General information: TITO was launched using: RESULT: Template: 4GKW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 18 1121 62.25 7.37 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 62.25 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_4GKW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GKW-query.scw PDB file : Tito_Scwrl_4GKW.pdb: