Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MILRHLGISPTNDLVAKKIFSNPEITCQFIRDML-DLPAKNVTILE---GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVH--HQNFFINHLWAYLCSQVNQNLEKIHQREGDTH--------- 2M89 Chain:A ((1-134)) MVNINHRIGIKASPEKIYQALTTDDGLKKWWTNDISGAGVVGSTIKFRFNGGGPDFKVTKLIPNKTVCWQHAGNMPESWMGTEISFQLETVENQTFVRFTHSNWHETTDFMAHCNTKWAVFLLSLKDALEIGKG

General information: TITO was launched using: RESULT: Template: 2M89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 457 10157 22.22 86.81 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 22.22 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_2M89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M89-query.scw PDB file : Tito_Scwrl_2M89.pdb: