Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQ-GQLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQR-GAIFSATSDSEILAHLIRRSHNP---------SLMGKIKEALSLVKGGFAYILLFE-DKLIAALDPNGFRPLSIGKMAN----GAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYD-SYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFK-----HEADIVVGVPNSSLSAAMGFAEEAGLPNEMGLIKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK 1ECF Chain:B ((1-473)) -----------CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVFEA-RHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNS-PYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAEN-PDIQQFECSVFNGVYVTKDVDQG---------------

General information: TITO was launched using: RESULT: Template: 1ECF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2774 -22523 -8.12 -49.94 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -8.12 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1ECF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECF-query.scw PDB file : Tito_Scwrl_1ECF.pdb: