Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLSIAISSYNAAAYLHYCVESLVI-GGEQVGILIINDGSQDQTQEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKILETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQYTMMHSLIYRTDLLRASQF 2Z86 Chain:B ((375-538)) -PLVSIYIPAYNCSKYIVRCVESALNQTITDLEVCICDDGSTDDTLRILQEHYANHPR-VRFISQKNKGIGSASNTAVRLCRGFYIGQLDSDDFLEPDAVELCLDEFRK----DLSLACVYTTNRNIDREGNLISNGYNWPIY----------SREKLTSAMICHHFRMFTARAWNLTE-

General information: TITO was launched using: RESULT: Template: 2Z86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 765 -44018 -57.54 -271.72 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -57.54 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2Z86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z86-query.scw PDB file : Tito_Scwrl_2Z86.pdb: