Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQ---VGLKEVANYLGEIFKRVGAEVEIDES-----------YTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKL-RGADLLVWEQGTKNALEQLEISGGNKGIVTFDAK--VKSADVDIHSSYGG-VVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGF-D--KVELYYTL--GEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHT-VFD-ALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE 2ZOG Chain:A ((8-474)) ------LKAVFQYID-ENQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMMEVAAADVQRLGGSVELVDIGKQKLPDGSEIPLPPILLGKLGSD-PQKKTVCIYGHLDVQPAALEDGWDSEPFTLVEREGKLYGRGSTDDKGPVAGWMNALEAYQKTGQEIPVNLRFCLEGMEESGSEGLDELIFAQKDKFFKDVDYVCISDNYWLGKNKPCITYGLRGICYFFIEVECSD--KDLHSGVYGGSVHEAMTDLISLMGCLVDKKGKILIPGINDAVAPVTDEEHALYDHIDFDM-EEFAKDVGAETLLHS-CKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPDMIPEVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQAGRRALKTVFGV-EPDLTREGGSIPV-TLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRLNYIEGTKMLAAYLYEV-

General information: TITO was launched using: RESULT: Template: 2ZOG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2274 50721 22.30 114.75 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 22.30 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_2ZOG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZOG-query.scw PDB file : Tito_Scwrl_2ZOG.pdb: