Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKNIYFVVLDLHTT-DRDKIIQLFKDWTDYSAKLVEGELVKKDGQNALFPPSDTGETV--GLNPHRLTLTFGVSASFLKRMNLENKRPRLFRDLPLFPKEQLREKYTGGDIVIHACADDEQIAFHAIRNLIRKGRNAVPLRWSQSGFAAIGD---RMETPWNLFGFKDGTANPTKEQ-DFDRVIWADS-KDWMENGSYMAVRRIQMFLETWDRTSLEEQENTFGRYKESGAPFGKKNEF---DEVDL--------SLLPDDSHVCLAKEV---DKPLLRRSYSYSDGIDEKTGQFDTGLLFISFQKDP-DNFVKVQTNLGATDKMNEYITHIGSGLFTCFGGVEKGGYIGQKLLEG 5MJH Chain:A ((27-376)) -QARGHLVAFDLAAGAGRKEAAALLRRWSDTARRLMAGEPAG-----------SRDTDVARDAGPSSLTVTFGFGHSFFGRTGLEKQRPVALDPLPDFSSDHLDKNRSNGDLWVQIGADDALVAFHALRAIQRDAGAAARVRWQMNGFNRSPGATAHPMTARNLMGQVDGTRNPKPGEADFDRRIFVPEGPAWMANGSYVVVRRIRMLLDDWEELSLKAQEDVIGRRKSDGAPLSGGSGATESTEMDLEKTDGSGELVVPINAHARITRPDQNGGAAMVRRPFSYHDGFDAD-GVPDAGLLFVCWQADPLRGFVPVQRKLDRGDALSQFIRHEASGLFAVPGGAAEGEYVGQRLLE-

General information: TITO was launched using: RESULT: Template: 5MJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1610 31665 19.67 98.95 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 19.67 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_5MJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MJH-query.scw PDB file : Tito_Scwrl_5MJH.pdb: