Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLLKETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEADLPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKPLIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPLFFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPRELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK 5FA9 Chain:B ((2-312)) IKEIYLAGGSFWGVEGYFRQIPGVKETDTGYAN-------------SDHAETVKIVYDSSVVSLQELLAHYFRIIDPTSLNKQGNDAGRQYRTGIYYVDDSMIKEINSFVKFMQKKYSRPIVVEVEKLKHFILAEDYHQDYLQKNPGGYCHIDLTLALKPLYDESKFKVPSKEELKKSLKPIQFSVTQEKATERPFTSEYDKFDAEGIYVDITTGKPLFSSLNKYDAGCGWPSFTKAITTQALQYLEDKSLGMNRTEVVSKTGGAHLGHVFDDGPADAGGLRYSINGAALRFIPYDKMEKEGYGDYLPYVK-

General information: TITO was launched using: RESULT: Template: 5FA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1614 -123752 -76.67 -415.28 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -76.67 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_5FA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FA9-query.scw PDB file : Tito_Scwrl_5FA9.pdb: