Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYGRGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRKITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANHVVVFGGKEIDVPENL-T--------VAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVVETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEILWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKATVERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY 1Q1L Chain:C ((6-398)) LRFLTAGESHGKGLTAILEGIPANLPLSEEEINHELRRRQRGY---------KDTAEILSGVRFGKTLGSPIALFIRNRDWE----------------------------ADLSGGIKYNQRDLRNILERASARETAARVAVGAVCKKFLSEFGIKIGSFVVSIGQKEVE-ELKDKSYFANPEKLLSYHEKAE-DSELRIPFPEKDEEFKTYIDEVKEKGESLGGVFEVFALNVPPGLGSHIQWDRRIDGRIAQAMMSIQAIKGVEIGLGFEAARRFGSQVHDEIGWSEGKGYFRHSNNLGGTEGGITNGMPIVVRVAMKPI-----------------------------VPAASVVGEAMLAIVLADALLEKLGGDFMEEVKKRFEDYVNHVKSF

General information: TITO was launched using: RESULT: Template: 1Q1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1701 -34887 -20.51 -109.02 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -20.51 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1Q1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q1L-query.scw PDB file : Tito_Scwrl_1Q1L.pdb: