Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKILVFGHQNPDSDAIGSSVAFAYLAKEA-YGLDTEAVALGTPNEETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRVANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTTHPTDKIIAPELAELAGVNLE-EYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRVAQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIV-NSNSEILA-LGANM-DKVEAAFNFKL--ENNHAFLAGAVSRKKQVVPQLTESFNA 1K20 Chain:B ((1-309)) -SKILVFGHQNPDSDAIGSSYAFAYLA-REAYGLDTEAVALGEPNEETAFVLDYFGVAAPRVITSAKAEGAEQVILTDHNEFQQSVADIAEVEVYGVVDHHRVANFETANPLYMRLEPVGSASSIVYRMFKEHSVAVSKEIAGLMLSGLISDTLLLKSPTTHPTDKAIAPELAELAGVN-LEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRVAQVNTVDIAEVLERQAEIEAAIEKAIADNGYSDFVLMITDIINSNSEILAIGSNMDKVEAAFNFVLENNHAFLAGA-----VSRKKQVVPQLTESFN-

General information: TITO was launched using: RESULT: Template: 1K20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1826 17908 9.81 59.30 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.91 3D Compatibility (PKB) : 9.81 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_1K20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K20-query.scw PDB file : Tito_Scwrl_1K20.pdb: