Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGA-RDGHDFIETAFENGAAVTLSEKEV-SNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAPADPIVEDYLPIDK----KVVRFGQG-AELEITDLVERKDSLTFKANFLEQALDLPVTGKYN---ATNAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDV-LDIVIFYGEDIAQLAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK 3ZL8 Chain:A ((2-422)) ---------------------KDLLTRRFVLNSKEVREGDVFVAVKGKRFDGHDFIDEALRNGAYAIIAERKTVNSDRIFLVESSVDTLAKLAREKLGNFSGTVVGVTGSSGKTTTKEILYNLLKNKRSVFKTPGNMNTEYGLPLSILNDYKGEEILVLEMAASRPGDIAHLCKIAPPDVAVLLNVGSAHLEFFGTRERIMETKMEIIKHSKENAIAVTLFDDP---DLRKEVPRYRNTLFFGKEGGDSVLKDWWYYEGSTIAEFEAFDSLFTVKLSGYWNGGQLLNIAASLCVMRTLGETVDI--FDLASLKTVPGRFNVREK-KGVLIVDDTYNASPEAFQTSIEALLRF----PGKKFAVVGAMKELGERSKEFHEELGERL-NVLDGVYVFLSEPEAEWIKSKK-------IILKSD--D-P---EKIAKDLATRVKKGDVVLFKASRAVRIERVLEMFEKE--

General information: TITO was launched using: RESULT: Template: 3ZL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2420 59496 24.58 145.11 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 24.58 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_3ZL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZL8-query.scw PDB file : Tito_Scwrl_3ZL8.pdb: