Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVDRVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLLDMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGDKVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS 5HYT Chain:A ((9-37)) --------------------------------------------------------------------------------ISQESKLINTLTDENEKLREELQQYYALS-------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5HYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3 586 195.17 20.19 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : 195.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.804

(partial model without unconserved sides chains): PDB file : Tito_5HYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HYT-query.scw PDB file : Tito_Scwrl_5HYT.pdb: