Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLYLLKLELFWFELHQHYHFSEPQKILNELIAAYSAAYSEKQRAYHTVQHLYECLSLIETVQSE--------LNDPYAVALALWFHDVVYEPQA------------------LDNELKSAELFEQLMAQ-DL------QLD----TMQKIKRWILATQKHAPT-----------------------------DETDLQFLLDIDLAILAATPERFMQYEQQIQQEYSWADPEVYSIKRKEVLMNFYQSEPLYQTAYFQKNFELNAKQNLKKILE 1Y2K Chain:A ((89-277)) ----------------------------------------YHA-DVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFF-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Y2K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 404 1711 4.24 13.91 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 4.24 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1Y2K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y2K-query.scw PDB file : Tito_Scwrl_1Y2K.pdb: