Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYIWMPETNGVWHWSNGENWLQAASLDQLIQDLQIHQGKEAAVFFPSRHAQMLQQTMAKFHYKQLGADGVKYLLEEFVTLPIDHMKVVHHFHADQLTVLAVAQGMVETWQHSLALLPTKLVALLPDFLVLPEPQAQQVILCNIGHQLLVRENKWLGNSIDDLGLFLEFQSAETHYQYSGLTAEQLESLEAASSAEQRSEFVYQFQPLDKTKQHPFNVLPKSKGQERTFSNYWKACAAVVLAIIVVQFSYDLLRWVKLKKVADQTAEQAIEQYKYWFGSSSGRVTEQNVRSNFKSRLEVSQQGDTQALSLLSRVGPILMQRQILAQQLNYDASILTMALKTKSADDLQALTQQLNQQGFKAELGNVQADGSGAIGVVKIQ 5J9T Chain:C ((8-279)) ------------------------------------MTWNEYDKFYTGSFQE--TTSYIKFSATVEDCCGTNYNMDERDETFLNEQ--VNKGSSDILT------------------------------------EDEFEILCSSFEHAIHERQPFLSMDPESILSFEELKPTLIKSDMADFNLRNQLNHEINS---HKTHFITQFDPVSQMNTRP-------------------------LIQLIEKFGSKIYDYWRERKIEVNGYEI-FPQLKFERPGIDPYVC-------FRRREVRHPRKTRRIDILNSQRLRALHQELKNAKDLALLVAKRENVSLNWINDELKIFDQRVKIKNLKRSLNISGEDDDLINHKRKRP

General information: TITO was launched using: RESULT: Template: 5J9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 597 -38996 -65.32 -145.51 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain C : 0.60 3D Compatibility (PKB) : -65.32 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_5J9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J9T-query.scw PDB file : Tito_Scwrl_5J9T.pdb: