Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGKILNKFKAWYKGDPGDMRWDPRTDTYVGTREPSKHWTAKVLSYF--------VDFSLLIAKSIKKHPSAYITQLLAFIAILVSCFSIYLQYYVDDDEYKRCTIAHTNNQEIALKCKK-------------- 1G3P Chain:? ((92-217)) EYGD--TPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTYYQYTP--VSSKAMYDAYW--NGKFRDCAF-HSGFNEDIFVCEYQGQSSDLPQPPVNA

General information: TITO was launched using: RESULT: Template: 1G3P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 368 17834 48.46 169.84 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 48.46 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.014

(partial model without unconserved sides chains): PDB file : Tito_1G3P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G3P-query.scw PDB file : Tito_Scwrl_1G3P.pdb: