Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMNMPVQHILQSVDWSKYSFEEWCRQLGAWLNGDTEIMVKIVKTMPTKRITQKQREKLIAMYMSDENLKDRLCTRRKGTCCQLNDNEARALHRLIIDIKLIEDHILQDWISAIWSHHVMGNSLRDIAQSNDTSVN----QIRQDLKCGLAYIKSRNPQFKFETFEKTA 5UEB Chain:A ((6-147)) ---HHHMKSKLTVVYYDLES------------NIAEEILSG--NIMPDGNFLIQE----IPLFAPNLALNDIVAIEREDK-MLFFDHLIKASGNTTINIVVL-DHFPKDLLAAIEEH----SGKIRKNGENYLSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS

General information: TITO was launched using: RESULT: Template: 5UEB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 633 -17502 -27.65 -126.83 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -27.65 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_5UEB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UEB-query.scw PDB file : Tito_Scwrl_5UEB.pdb: