Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKLCDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQG-KDLQQVLADLKTLLTDNGFVVDYVE-ARQPNLLA--ASQFDRD--IVLFVAAKLGGTRLIDNLQVAFTPQ 3UK2 Chain:B ((5-283)) MKVISSIQELRDQLR----GQNRTAFVPTMGNLHEGHLSLMRLARQHGDPVVASIFVNRLQFGPNEDFDKYPRTLQEDIEKLQKENVYVLFAPTERDMYPEPQEYRVQPPHDLGDILEGEFRPGFFTGVCTVVTKLMACVQPRVAVFGKKDYQQLMIVRRMCQQLALPVEIVAAETVRDADGLALSSRNRYLSEAERAEAPELAKTLARVRDAVLDGERDLAAIERRAVAHLSARGWQPDYVSIRRRENLVAPSAAQIEAGDPLVVLTAAKLGATRLIDNLEI-----

General information: TITO was launched using: RESULT: Template: 3UK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1340 -128990 -96.26 -472.49 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -96.26 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_3UK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UK2-query.scw PDB file : Tito_Scwrl_3UK2.pdb: