Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKYSNINSFNALQTLTVGSSNYQIFSLTQAEKKLGDIAKLPKSLKVLLENLLRFEDQHSVKTEHIYALAEWLKTRTSDQEIQYRPARVLMQDFTGVPAVVDLAAMRAAMAQAGGDPEKINPLSPVDLVIDHSVMVDHFADDQAFEENVQIEMQRNGERYQFLR-WGQSAFNNFSVVPPGTGICHQVNLEYLAQAVWLGEDNGQTFAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPISMLIPEVIGFKLTGKLQEGITATDLVLTITQMLRQKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPIDEVTLGYLKLTGRQSDRIALVEAYSKAQGLWRNAGDEPVFTDTLSLDMSTVQASLAGPKRPQDRVLLSEVPKTFNALMELTLKPAKEAKERLENEGGGGTAVEATKANIQHESPSCVIEGQEYPLNHGDVVISAITSCTNTSNPSVMLAAGLLAKKAIEKGLQRKPWVKSSLAPGSKVVTDYLLAAGLTPYLDELGYNLVGYGCTTCIGNSGPLPEPIEDAIQCHDLNVASVLSGNRNFEGRVHP-LVKTNWLASPPLVVAYGLVGNIRTDLTTQPIGQGKDGQPVYLKDIW-PSQAEIDAVLQKVNTDMFHKEYAAVFDGDESWQAIQIPKSKTYEWADDSTYIRHPPFFEGIGEPPKPIKNIEQARILAVLGDSVTTDHISPAGNIKKDSLAGRYLQEQGVEPKDFNSYGSRRGNHEVMMRGT-FANIRIKNEMLGGEEGGNTIHVPSGEKLAIYDAAMRYQHEHTPLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFVDGQTRQSLNLTGHEVISIRGLSDGIQPHEILEVDVKGPNGVASHFNVLC-RIDTLNEVEYFKAGGILHYVLRNLIAS 1B0K Chain:A ((33-752)) --------------------------------------RPLTLSEKIVYGHLDDPA------NQEIE-------R--GKTYLRLRPDRVAMQDATAQMAMLQFISSG-------LP--KV--AVPSTIHCDHLIEAQLGG-----EKDLRRAKDINQEVYNFLATAGA--KYGVGFWRPGSGIIHQIILENYAYPG-------------VLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADI-ANLADEF--KDHLVPDP--GCHYDQVIEINLSELKPHINGPFTPDLAHPVAEVGSVAE------K------------------------------------EG-----WPLDIRVGLIGSCTNSSYEDMGRSA-AVAKQALAHGLKCK--SQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRK------DIK-KGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTG-KDGKKF---KLEAPDADELPRA--EFDPG--QDTYQHPPKDS-SGQR--------VDVSPTSQRLQLLEPFDKW-----DGKDLEDLQILIKVKGKCTTDHISAAGP---------WLK-----------F---RGHLDNISNNLLIGAINIENRKA-----NSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGASREHSALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNK--IHPVDKLTIQGLKD-FAPGKPLKCIIKHPNG--TQETILLNHTFNETQIEWFRAGSALNRMKELQQ--

General information: TITO was launched using: RESULT: Template: 1B0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4653 20291 4.36 28.38 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 4.36 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_1B0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B0K-query.scw PDB file : Tito_Scwrl_1B0K.pdb: