Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKFKLPLAIAIASAVLVGCSSNKVKEAKPNPLPKLTESNKSLVPVFSRSVSSTNKADPLRLQLDASEGVVFTLDPKGEVAAYRGK--QRLWEKKVSKLG---------LSSGVEAAEGIVVVGNSKGQLFALDQATGEQKWTAQLSGALLSPSLVQSGRVITIANDGTVFAHDVSSGQQVWAYKLPNVQFSLRGQPSPVSLDPRTVLIASANAYVYAIDTISGIPHFQRRVAVSEGRSDIQRLIDIDGDPVVAGQLMVTTSFQGQVTVTDLASQRVVWSEDSSSTKRPEVYDNKVFVSSTDGKLTAYDLTTGEQLWQNDSLLNRHLSNPVVLGSDLIVGDLDGVLHLIDPTTGKLIGRSKTSGEVNTLRVIEKQLYVSTRKGDLSIWQNR 3P1L Chain:A ((32-362)) -----------------------------KMSPLPTV-ENQFTPTTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDGWFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPTTAFGAAV-VGGDNGRVSAVLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQIMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYNGNLVVGDSEGYLHWINVEDGRFVAQQK------------------------------

General information: TITO was launched using: RESULT: Template: 3P1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1829 511 0.28 1.60 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 0.28 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_3P1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P1L-query.scw PDB file : Tito_Scwrl_3P1L.pdb: