Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLINTEVKPFQATAYHNGQFIEVNETNLKGKWSVVFFYPADFTFVCPTELEDLADNYAEFQKLGVEIYGVSTDTHFTHKAWHDTSDAIKKIQYPLIGDPTWTLSKNFDVLIESEGLADRGTFVIDPEGKIQIVEINAGGIGRDASELLRKVKAAQYVHSHPGEVCPAKWKEGEATLAPSIDLVGKI 4MA9 Chain:D ((1-186)) -SLINTKIKPFKNQAFKNGEFIEVTEKDTEGRWSVFFFYPADFTFVCPTELGDVADHYEELQKLGVDVYSVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTGALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAAHPGEVCPAKWKEGEATLAPSLDLVGKI

General information: TITO was launched using: RESULT: Template: 4MA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 871 -88353 -101.44 -475.02 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.94 3D Compatibility (PKB) : -101.44 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_4MA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MA9-query.scw PDB file : Tito_Scwrl_4MA9.pdb: