Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVTAIVKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEIAISDEMVDAVIESITRVASTGKIGDGKIFLTNLEQVIRIRTGETGPDAV 3MHY Chain:B ((1-112)) MKLVMAIIKPFKLDEVREALTSLGIQGLTVSEVKGFGRQKGQTEIYRGAEYSVSFLPKVKVEVAVSDDQYEQVVEAIQKAANTGRIGDGKIFVLDIAQAVRIRTGETNTEAL

General information: TITO was launched using: RESULT: Template: 3MHY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 447 -58291 -130.40 -520.45 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.94 3D Compatibility (PKB) : -130.40 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_3MHY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MHY-query.scw PDB file : Tito_Scwrl_3MHY.pdb: