Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKILVTGGAGYIGSHTCVELLEAGHEVIVFDNLSNSSKESLNRVQEITHKSLTFVEGDIRNSGELDQVFQEHA-IDAVIHFAGLKAVGESQEKPLIYFDNNIAGSIQLVKSMEKAGVYTLVFSSSATVYDEA----NTSPLNEEMPTGMPSNNYGYTKLIVEQLLQKLSVAD-------------SKWSIALLRYFNPVGAHKSGRIGEDPQGIPNNLMPYVTQVAVGRREKLSIYGNDYDTIDGTGVRDYIHVVDLANAHLCALNN-RLQAQGCRAWNIGTGNGSSVLQVKNTFEQVNGVPVAFEFAPRRAGDVATSFADNARAVAELGWKPQYGLEDMLKDSWNWQKQNPNGYN 4LIS Chain:B ((6-357)) ---VLVTGGTGYIGSFTTLALLEAGYKVVVADNLYNSSAEALNRIELISGKKAEFAQLDVTDEAAFDKVFEAHPDIDSVIHFAALKAVGESGEKPLDYYHVNVYGTICLLRSMVRHNVTNIVFSSSATVYGDATRFPDMIPIPEHCPLG-PTNPYGNTKFAIELAITDVINAQRNNAKKAGNETEAAKWNGALLRYFNPAGAHPSGIMGEDPQGVPYNLLPLLAQVATGKREKLLVFGDDYASHDGTAIRDYIHILDLADGHLKALNYLRANNPGVRAWNLGTGRGSTVYEMIRAFSKAVGRDLPYEVAPRRAGDVLNLTSNPTRANTELGWKAQRTLEQACEDLWLWTKNNPQGY-

General information: TITO was launched using: RESULT: Template: 4LIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1820 -47315 -26.00 -142.09 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -26.00 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4LIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LIS-query.scw PDB file : Tito_Scwrl_4LIS.pdb: