Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVRRPTMSFAFWSRLKALVRKETKQLLRDKGSLGIGLVLPVILILLFGYGLSFDLNQARVGVVVDQSSPQVNQVLAGLNGSRYLTSLEFRNLPEAEQAIRNGKIDA-----ILHLPS-DFANQVQQGNAKVQLLLNGRSTTIATALEGY----VAGAL--ATAPSIQIDRSPILASASAVKI-------EQRIWFNE---SGNSTWFLVPGLM----ILILTLIGAFLTGLLIARERERGTLEALFVTPVRPFEIVLAKLIPYVVVGMIDIVICIVAAHFIFEVPMRGSLFSILFASFLYLIVSLLLGLTISGFAQSQFQASQIALLASFMPALMLSGFVFDTRNLPLVVQIISQLLPATHFMVL--IKTLFMGGDDWKLWFKECGILLGYIVVLICAVNFSLKKRLR 5TZJ Chain:A ((1-349)) MKFSVIVPTYNSEKYITELLNSLAKQ--------------------------DFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEH-HSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSD-----KAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEIL------------------NADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAV----------PERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGID

General information: TITO was launched using: RESULT: Template: 5TZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1758 -30202 -17.18 -94.09 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -17.18 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_5TZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TZJ-query.scw PDB file : Tito_Scwrl_5TZJ.pdb: