Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHA----FAEKTGVDLNYQKRLAPLDGFESSMRS-FFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTLWMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVI--ANRTLARAEQLVDDLKTAVPQAQLQAISLNDLEGDFDIVINATSASLSGDALQLPEKLK-----FKYAYEMAYGKPSSFLDQAKQRNVPYAEGFGMLVGQAIEAFSIWNGVRPQLKDFL 4FR5 Chain:B ((4-246)) --KSFGVFGNPIKHSKSPLIHNACFLTFQKELRFLGHYHPILLPL---ESHIKSEFLHLGLSGANVTLPFKERAFQVCDKIKGIALECGAVNTLVLENDELVGYNTDALGFYLSLK--QKNYQNA--LILGAGGSAKALACELKKQGLQVSVLNRSSRGLDFFQRLGCDCFMEPPKSA------------FDLIINATSASLHNELPLNKEVLKGYFKEGKLAYDLASGFLTPFLSLAKELKTPFQDGKDMLIYQAALSFEKFS----------

General information: TITO was launched using: RESULT: Template: 4FR5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1279 9611 7.51 41.61 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 7.51 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.360

(partial model without unconserved sides chains): PDB file : Tito_4FR5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FR5-query.scw PDB file : Tito_Scwrl_4FR5.pdb: