Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MALVLDGRALAKQIEENLLVRVEALKAK-TGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELPQETTTEQLLAEIEKLNANPDVHGILLQHPVPAQ--IDERACFDAISLAKDVDGVTCLGFGRMAMGE--AAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAMMLLQANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGV-GDIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG 1A4I Chain:A ((2-296)) APAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINY----KVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLE

General information: TITO was launched using: RESULT: Template: 1A4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1580 35114 22.22 126.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 22.22 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_1A4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4I-query.scw PDB file : Tito_Scwrl_1A4I.pdb: