Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTLDEMTTVTDDQSELTMSVLMTPDMANFSGNVHGGTILKLLDQVAYACASRYSGSYVVTLSVDKVNFKEPIYVGELVTFLASVNHVGRTSMEVGIRVEAQNIQKRTVRHTNSCYFTMVAVDEHGKPRKVPKLNLDTEWKRCRFEAAEHRKVLRLQENHNPSCSMYKKTS 5T02 Chain:F ((14-154)) ---------------ELIMSELMMPDTANFSGNVHGGELLLLLAQVAYSCASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTGEIRHTNSCYFTMVAVKD-GKPVPVPPLEILTDRQRCRYEKAKKRRDISLQ--------------

General information: TITO was launched using: RESULT: Template: 5T02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 626 -87588 -139.92 -621.19 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain F : 0.83 3D Compatibility (PKB) : -139.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_5T02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T02-query.scw PDB file : Tito_Scwrl_5T02.pdb: