Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFIVFHFYKGKIMRAYNFCAGPAALPTAVLEKAQQELLDWQGKGLSIMEMSHRSADYVAVAEKAEADLRKLMNIPENYKVLFLQGGASLQFSAIPLNLLGKNNKADYIHTGIWSEKALKEAKRYG-DINVVEAGIKVDGKFAISEQSEWNLSDDAAYVHYADNETIGGLQF-AGVPDV-KAPLVCDFSSSILSAPLDVSKFGLIYAGAQKNIGPAGLTIVIIRDDLLDQAKAEIPSILKYADQAKNGSMVNTPSTYAWYLSGLVFEWLLEQGGVDAIHKVNLEKAQLLYGYIDSS-DFYNNPIAIPNRSIMNVPFTLADKALEKQFLKEAEANHLLNLAGHRSVGGMRASIYNAVPLEGVQALIRFMDDFAKRNG 1W23 Chain:A ((1-359)) -------------VKQVFNFNAGPSALPKPALERAQKELLNFNDTQMSVMELSHRSQSYEEVHEQAQNLLRELLQIPNDYQILFLQGGASLQFTMLPMNLLTKGTIGNYVLTGSWSEKALKEAKLLGETHIAASTKA--NSYQSIPDFSEFQLNENDAYLHITSNNTIYGTQYQN-FPEINHAPLIADMSSDILSRPLKVNQFGMIYAGAQKNLGPSGVTVVIVKKDLLNTKVEQVPTMLQYATHIKSDSLYNTPPTFSIYMLRNVLDWIKDLGGAEAIAKQNEEKAKIIYDTIDESNGFYVGHAEKGSRSLMNVTFNLRNEELNQQFLAKAKEQGFVGLNGHRSVGGCRASIYNAVPIDACIALRELMIQFKEN--

General information: TITO was launched using: RESULT: Template: 1W23.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2023 -66351 -32.80 -186.90 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -32.80 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1W23.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W23-query.scw PDB file : Tito_Scwrl_1W23.pdb: