Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFKVKNV--DNYAGHI-DLGEGDETLGIFGHMDVVPAG--DGWDTDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGW-GDMAYYFEH----EEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTR-GARVMEPHYVPMDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLK----RGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 3ISZ Chain:B ((2-376)) ------------KEKVVSLAQDLIRRPSISPN------------DEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWSSPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCMVGEPSSAKN---LGDV---VK---------------------------------------------------------NGRRGSITGNLYIQGIQ-----PHLAENPIHKAALFLQELTTY------QW--DK---------------GNEFFPPTSLQIANIHAG--VIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKP-GKLLDSITSAIEETIGITPKAETGGGTSDGRFIALMGAEVVEFGPLNS----TIHKVNECVSVEDLGKCGEIYHKMLVNLLD

General information: TITO was launched using: RESULT: Template: 3ISZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2007 52522 26.17 150.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 26.17 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_3ISZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ISZ-query.scw PDB file : Tito_Scwrl_3ISZ.pdb: