Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGKVSDIEGLIEK---GPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYSLRLAQEISYIYGFKDLMGENGELS--EEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLSMKPMATKLQTELGKNVAYTEESLQQDLDILEGEFEEVSSDIASSDTVLSQLERLSHLLETNMITEEEFQQLKQELLKK 4D10 Chain:G ((12-219)) --------------------TGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPNVRELAE-----------------------------SDFASTFRLLTVFAY-GTYADYLAEARNLPPLTEAQKNKLRHLSVVTLAAK----------------VKCIP---------YAVLLEALALRNVRQLEDLVIEAVYA------DVLRGSLD---QRNQRLEVDYSIGRDIQRQD---------LSAIARTLQEWCVGCEVVLSGIEEQVSRANQHKEQQLGLKQQIESEVAN

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 665 -6745 -10.14 -33.23 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain G : 0.62 3D Compatibility (PKB) : -10.14 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: