Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEPEPEKKLQELADKILLE-DYEVIALQEINQLLESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEH---GKEYFWSWEMSHIGYDKYEEGNALLTKKTLES-QVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKSGFYFEWKNLENYFL------ETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKAATVEKKIDGWEQNTEKLRIDFIFVPEGMQVK--KYQ--RIFDGIDSPIISDHYGVEIELDVNE 2F1N Chain:A ((-1-251)) --TDLTDFRVATWNLQGASATTESKWNINVRQLISGENAVDILAVQEAGSPPSTAVDTG---RVIPSP--------GIPVRELIWNLSTNSRPQQVYIY-FSAV--DALGGRVNLALVSNRRADEVFVL--SPV----RQGGRPLLGIRIG----NDAFFTAHAIAMRNND--APALVEEVYNFFRDSRDPVHQALNWMILGDFNREPADLEMN-LT-V--PVRRASEIISP--AAAT-------Q---TSQRTLDYAVAGNSVAFRPSPLQAGIVYGARRTQISSDHFPVGVSRR---

General information: TITO was launched using: RESULT: Template: 2F1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1329 35084 26.40 148.03 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 26.40 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_2F1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F1N-query.scw PDB file : Tito_Scwrl_2F1N.pdb: