Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFQIQYKSSTRDGGNNVNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGIPIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSSLAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDSEGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN 3UWO Chain:B ((11-201)) -------------------GLFVTLEGPEGAGKSTNRDYLAERLRERG-IEVQLTREPGGTPLAERIRELLLAPSDEPMAADTELLLMFAARAQHLAGVIRPALARGAVVLCDRFTDATYAYQGGGRGLPEARIAALESFVQGDLRPDLTLVFDLPV--------------LDRFEQEDRRFFEAVRQTYLQRAAQAPERYQVLDAGLPLAEV------------------

General information: TITO was launched using: RESULT: Template: 3UWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 793 -60459 -76.24 -337.76 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -76.24 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_3UWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UWO-query.scw PDB file : Tito_Scwrl_3UWO.pdb: