Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIARHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVREVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYNKFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDLYECTEMMLIRNFGK-MGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLTQLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLIWEEILG-VEKGIRLLGITLTNLDPMTYENIVLPLWENQEI 3RAX Chain:B ((1-341)) -------------GIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEARKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIEIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGINKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRT-RVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRI--PKAIHVVAVTEDLDIVSRGRTFPHGIS-KETAYSESVKLLQKILEEDERKIRRIGVRFSKFI-----------------

General information: TITO was launched using: RESULT: Template: 3RAX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1656 -141228 -85.28 -416.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -85.28 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_3RAX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RAX-query.scw PDB file : Tito_Scwrl_3RAX.pdb: