Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MIYKG----------------TLLYKPEECSHCLCVVPSRIIRWGT------------TTVRLLLNDVSEYRTYL---ELKKQRFKCKSCQRT-----FVADTSVA--------------------EKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVY--RVMKRFYRP-LNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTF------LNHRVKETTVRLKGNSHSQRGLGKN 4QTZ Chain:A ((6-326)) LGNVVCVTGASGYIASWLVRLLLHRGYTVKATVRDPNDPKKVDHLVKLDGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASPFYHDVKDPQAELIDPALKGTLNVLNSCAKSPSLKRVVLTSSIAAVAYNGKPRTPDVVVDETWFTDADFCAKSNLWYVVSKTLAEEAAWKFVKENNIDMVTINPAMVIGPLLQPVLNTSAAAILNLI--NGAQTFPN--ASFGWVNVKDVANAHILAYENASAS--GRHCLVERVAHYSEVVRILRELY-PSLQLPEKC-ADDKPYVPIYQVSKEKAKSLGLEYTPLEVSIKETVESLKEKKFANL-----

General information: TITO was launched using: RESULT: Template: 4QTZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1155 31274 27.08 133.08 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 27.08 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_4QTZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QTZ-query.scw PDB file : Tito_Scwrl_4QTZ.pdb: