Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MLYDL-----------------------LQTKHIYIVCGKTDLRKGID-GLASLIQQEYQL--ELYEDAVFLFCGNRQDRFKLLYWDGDGFLL--CYKRIENGKLKWPRTKDEVRTLTNQP-----VKWLLEGLSIDQPRAILPGKKGVF----------------------- 1Z8K Chain:A ((20-193)) KVQELSVYEINELDRHSPKILKNAFSLMFGLGDLVPFTNKLYTGDLKKRVGITAGLCVVIEHVPEKKGERFEATYSFYFG----DYGHLSVQGPYLTYEDSFLAITGGAGIFEGAYGQVKLQQLVYPTKLFYTFYLKGLANDLPLELTGTPVPPSKDIEPAPEAKALEPSGVISNYTN

General information: TITO was launched using: RESULT: Template: 1Z8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 474 5930 12.51 52.47 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 12.51 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_1Z8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z8K-query.scw PDB file : Tito_Scwrl_1Z8K.pdb: