Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQKRQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALGVDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGLKVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGP---------------REWYDDEFEVYGHYMPLDELLDQVDVMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADSLVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA 5G1O Chain:C ((56-311)) --------------------------------------------------SMFYEVSTRTSSSFAAAMARLGGAVLSF--------KGESLADSVQTMSCYA-DVVVLRHPQPGAVE--LAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFE------SIEEALPDTDVLYMTRIQKERF-----------FGQFILTPHIMTRAKKKMVVMHPMP--RVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVL----

General information: TITO was launched using: RESULT: Template: 5G1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1113 -76735 -68.94 -342.56 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -68.94 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_5G1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1O-query.scw PDB file : Tito_Scwrl_5G1O.pdb: