Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPELAKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIRGDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK 1BE4 Chain:C ((4-135)) -ERTFIAIKPDGVQRGLMGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIALWFRP------------

General information: TITO was launched using: RESULT: Template: 1BE4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 653 -88944 -136.21 -673.81 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -136.21 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1BE4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BE4-query.scw PDB file : Tito_Scwrl_1BE4.pdb: