Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGASDKLLREPFRANFKRERSSSYSTERGSPTSPHLRRLRPLPTSSSEGKGAADGDGEEDPHTRRQAEPELFELTPHGGTANTNVRATRFGVEGLRIGRDPSCCDLVLPSNSVSRLHCVLSVLGDDVFVHDNSFNGTFINGRRVGRGRCSVLHPRDTLSFLNPTLEEASRCGFEFAPLPGHSSSAFTAVEGLRRYELGPVLGQGSLAAVRLGIDRETGAPVAIKLIERGRFS-----CEEAAASLHTEIEMMRSM-DHPHVVRVVDAFEGSGCVALVMEYVRGGDLFDYIVGRGRNPFTEAEARHLFGQLLEAILYIHGRSIIHCDLKPENVLVDVVRRGSDGEVDTTSASATATTQGVASSALQTDGDAALSVVDDHQAEAKTVSPYDVRLKLADFGSAKYEGGGAGGGMLETTGAATPVYAAPELACFPADGAPPHEITAVVDVWSLGVLLYILCSGTVPKPPRADAVV---AFNR-------SMTHLSVLCKDLIARMMTVDPSQRPSLADVCHHPWLDGLTISGAPDRGALGSKDVLSVTAKLSPSFPEAAKPL 2Y7J Chain:D ((91-364)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEV---RLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKV--ALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDD----------------------------------------------------NMQIRLSDFGFSCHLEPGE----KLRELCGTPGYLAPEILKCSMD-ETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFE------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1153 -26002 -22.55 -101.97 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -22.55 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_2Y7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y7J-query.scw PDB file : Tito_Scwrl_2Y7J.pdb: