Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGILALQGAFAEHAKVLDQLGV---ESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQ--NMLLPIREAI-LSGLPVFGTCAGLILLAKEITSQKE-------SHLGTMDMVVERNAYGRQLGSFYTEAECKGV-----GKIPMTFIRGPIISS-VGEGVEILAIVN------NQIVAAQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS 2ABW Chain:B ((4-216)) ITIGVLSLQGDFEPHINHFIKLQIPSLNIIQVRNVHDL-GL---CDGLVIPGGESTTVRRCCAYENDTLYNALVHFIHVLKKPIWGTCAGCILLSKNVENIKLYSNFGNKFSFGGLDITICRNFYGSQNDSFICSLNIISDSSAFKKDLTAACIRAPYIREILSDEVKVLATFSHESYGPNIIAAVEQNNCLGTVFHPELLPHTAFQQYFYEKVKNY-

General information: TITO was launched using: RESULT: Template: 2ABW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 911 -44821 -49.20 -238.41 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -49.20 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_2ABW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ABW-query.scw PDB file : Tito_Scwrl_2ABW.pdb: