Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQALFGGGCFWCVEAVFLQIRGVEKVTSGYAGGHTTHPTYEQVCQGDTQHAEVVLIDFDEQQVTYSQLLDVFFATHDPTTLNRQGNDIGTQYRSVIYYFNEEQKQAAEHT---IQTLK-----DDDLDIVTELSPA-----PTFYPAEDYHQNYYEKNPSQGYCNFAIPPKLLKLYSKFQHLMKDQ 4D7L Chain:B ((63-232)) TETIYIGIGCYWGAEKLFWETPGVVYTSVGFAGGITPNPTYRETCTGRTNHTEIVEVVYDPTQVTFDELVVKAMEAHDPTQGYRQGNDTGTQYRSAIYTAGPNAEQQAQRAREIVEHYAPKLAAAGLGRITTEILPLASTPAGEYYMAEDEHQQYLHKNPL-GYCPHHSTG----------------

General information: TITO was launched using: RESULT: Template: 4D7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 818 -47881 -58.53 -304.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -58.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_4D7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D7L-query.scw PDB file : Tito_Scwrl_4D7L.pdb: